KSR2 antibody (Internal Region)

Catalog No. ABIN6257612

This Rabbit Polyclonal antibody specifically detects KSR2 in ELISA, WB, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for KSR2 antibody (Internal Region) (ABIN6257612)

Target: KSR2 (Kinase Suppressor of Ras 2 (KSR2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KSR2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-KSR2 Antibody

Binding Specificity: Internal Region

Specificity: KSR2 Antibody detects endogenous levels of total KSR2.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human KSR2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for KSR2

Target: KSR2 (Kinase Suppressor of Ras 2 (KSR2))

Alternative Name: KSR2

Background:

Description: Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

Gene: KSR2

Molecular Weight: 108 kDa

Gene ID: 283455

UniProt: Q6VAB6