BUB1B antibody (C-Term)

Catalog No. ABIN6257686

This anti-BUB1B antibody is a Rabbit Polyclonal antibody detecting BUB1B in WB, ELISA, IF and ICC. Suitable for Human and Mouse.

Quick Overview for BUB1B antibody (C-Term) (ABIN6257686)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BUB1B Antibody

Binding Specificity: C-Term

Specificity: BUB1B Antibody detects endogenous levels of total BUB1B.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BUB1B, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B

Background:

Description: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Gene: BUB1B

Molecular Weight: 130 kDa

Gene ID: 701

UniProt: O60566