GRK6 antibody (N-Term)

Catalog No. ABIN6258200

This Rabbit Polyclonal antibody specifically detects GRK6 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GRK6 antibody (N-Term) (ABIN6258200)

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GRK6 Antibody

Binding Specificity: N-Term

Specificity: GRK6 Antibody detects endogenous levels of total GRK6.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GRK6, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GRK6

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Alternative Name: GRK6

Background:

Description: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

Gene: GRK6

Molecular Weight: 65 kDa

Gene ID: 2870

UniProt: P43250

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity