CD28 antibody (Internal Region)

Catalog No. ABIN625822

This Goat Polyclonal antibody specifically detects CD28 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CD28 antibody (Internal Region) (ABIN625822)

Target: CD28

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CD28 Antibody

Binding Specificity: Internal Region

Purpose: CD28

Sequence: SQQLQVYSKT GFNCD

Specificity: The immunizing peptide corresponds to a part of the extracellular domain.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: SQQLQVYSKTGFNCD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 48 kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary incuba

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28, CD28 Molecule, MGC138290, Tp44, CD28 antigen, CD28 antigen (Tp44), T-cell-specific surface glycoprotein

Gene ID: 940

NCBI Accession: NP_006130

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response