RORC antibody (AA 200-212)

Catalog No. ABIN625825

This Goat Polyclonal antibody specifically detects RORC in WB, ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for RORC antibody (AA 200-212) (ABIN625825)

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RORC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-RORC Antibody

Binding Specificity: AA 200-212

Purpose: RORC (aa200-212)

Sequence: CHLEYSPERG KAE

Specificity: This antibody is expected to recognize both reported isoforms (NP_005051.2, NP_001001523.1).

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CHLEYSPERGKAE

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55-60 kDa band observed in Human Colon, Liver and Testis lysates (calculated MW of 55.8 kDa according to NP_001001523.1 and 58.2 kDa according to NP_005051.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Alternative Name: RORC

Background: RORC, RAR-related orphan receptor C, MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RAR-related orphan receptor C, isoform a, RAR-related orphan receptor gamma, nuclear receptor ROR-gamma, nuclear receptor RZR-gamma, nuclear receptor subfamily 1 group F me

Gene ID: 6097, 19885

NCBI Accession: NP_005051, NP_001001523

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway