PRDM14 antibody (AA 414-427)

Catalog No. ABIN625828

This Goat Polyclonal antibody specifically detects PRDM14 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for PRDM14 antibody (AA 414-427) (ABIN625828)

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRDM14 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PRDM14 Antibody

Binding Specificity: AA 414-427

Purpose: PRDM14 (aa414-427)

Sequence: YRDKHLKYTP CVDK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: YRDKHLKYTPCVDK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 40 kDa band in Human Heart lysates after 0.1-1 μg/mL antibody staining and this band was successfully blocked by incubation with the immunizing peptide. Preliminary experiments also gave an approxand an

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRDM14

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Alternative Name: PRDM14

Background: PRDM14, PR domain containing 14, MGC59730, PFM11, PR domain zinc finger protein 14, PR domain-containing protein 14, PR-domain zinc finger protein 14

Gene ID: 63978, 383491, 100365184

NCBI Accession: NP_078780