POU3F2 antibody (N-Term)

Catalog No. ABIN6258305

This Rabbit Polyclonal antibody specifically detects POU3F2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for POU3F2 antibody (N-Term) (ABIN6258305)

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POU3F2 Antibody

Binding Specificity: N-Term

Specificity: POU3F2 Antibody detects endogenous levels of total POU3F2.

Predicted Reactivity: Pig,Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POU3F2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2

Background:

Description: Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

Gene: POU3F2

Molecular Weight: 47 kDa

Gene ID: 5454

UniProt: P20265