Six5 antibody (C-Term)

Catalog No. ABIN6258326

This Rabbit Polyclonal antibody specifically detects Six5 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Six5 antibody (C-Term) (ABIN6258326)

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Six5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Six5 Antibody

Binding Specificity: C-Term

Specificity: SIX5 Antibody detects endogenous levels of total SIX5.

Predicted Reactivity: Bovine,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SIX5, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Six5

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Alternative Name: SIX5

Background:

Description: Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3 (By similarity).

Gene: SIX5

Molecular Weight: 75 kDa

Gene ID: 147912

UniProt: Q8N196