ZEB2 antibody (AA 545-558)

Catalog No. ABIN625833

This anti-ZEB2 antibody is a Goat Polyclonal antibody detecting ZEB2 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for ZEB2 antibody (AA 545-558) (ABIN625833)

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZEB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ZEB2 Antibody

Binding Specificity: AA 545-558

Purpose: ZEB2 (aa545-558)

Sequence: TDSRRQISNI KKEK

Specificity: This antibody is expected to recognize both reported isoforms (NP_055610.1, NP_001165124.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CTDSRRQISNIKKEK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100+38 kDa bands observed in Human Liver lysates (calculated MW of 136 kDa according to NP_055610.1). Recommended concentration: 0.2-0.5 μg/mL. Primary incubation was 1 hour. We believe the two observed bands represent two breakdown fra

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: ZEB2

Background: ZEB2, zinc finger E-box binding homeobox 2, FLJ42816, KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B, OTTHUMP00000204540, OTTHUMP00000204541, Smad-interacting protein 1, zinc finger E-box-binding homeobox 2, zinc finger homeobox 1b, zinc finger homeobox protein 1

Gene ID: 9839, 24136, 311071

NCBI Accession: NP_055610, NP_001165124

Pathways: Tube Formation