UBE2F antibody (N-Term)

Catalog No. ABIN625837

This Goat Polyclonal antibody specifically detects UBE2F in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for UBE2F antibody (N-Term) (ABIN625837)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-UBE2F Antibody

Binding Specificity: N-Term

Purpose: NEDD8 conjugating enzyme (N Terminus)

Sequence: SKLKRDDGLK GSRT

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SKLKRDDGLKGSRT-C, from the N Terminus of the protein sequence according to NP_542409.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 22 kDa band observed in Rat Pancreas lysates (calculated MW of 21.1 kDa according to Human NP_542409.1 and 18.6 kDa according to Rat NP_001008382.1). Recommended concentration: 0.05-0.2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, OTTHUMP00000202749, OTTHUMP00000202755, OTTH

Gene ID: 140739, 67921, 363284

NCBI Accession: NP_542409