ABHD2 antibody (Internal Region)

Catalog No. ABIN6258379

This anti-ABHD2 antibody is a Rabbit Polyclonal antibody detecting ABHD2 in ELISA, WB, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for ABHD2 antibody (Internal Region) (ABIN6258379)

Target: ABHD2 (Abhydrolase Domain Containing 2 (ABHD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABHD2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ABHD2 Antibody

Binding Specificity: Internal Region

Specificity: ABHD2 Antibody detects endogenous levels of total ABHD2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ABHD2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ABHD2

Target: ABHD2 (Abhydrolase Domain Containing 2 (ABHD2))

Alternative Name: ABHD2

Background:

Description: Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane (PubMed:26989199). Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA) (PubMed:26989199). Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation (PubMed:26989199). May also play a role in smooth muscle cells migration (By similarity).

Gene: ABHD2

Molecular Weight: 48 kDa

Gene ID: 11057

UniProt: P08910