POLR2J antibody (N-Term)

Catalog No. ABIN6258465

The Rabbit Polyclonal anti-POLR2J antibody is suitable to detect POLR2J in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for POLR2J antibody (N-Term) (ABIN6258465)

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2J antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR2J Antibody

Binding Specificity: N-Term

Specificity: RPB11 Antibody detects endogenous levels of total RPB11.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPB11, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR2J

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Alternative Name: POLR2J

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).

Gene: POLR2J

Molecular Weight: 13 kDa

Gene ID: 5439

UniProt: P52435

Pathways: Regulatory RNA Pathways