ATP5I antibody (C-Term)

Catalog No. ABIN6258537

The Rabbit Polyclonal anti-ATP5I antibody is suitable to detect ATP5I in samples from Human. It has been validated for ELISA, WB, IF and ICC.

Quick Overview for ATP5I antibody (C-Term) (ABIN6258537)

Target: ATP5I (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit E (ATP5I))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5I antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP5I Antibody

Binding Specificity: C-Term

Specificity: ATP5I Antibody detects endogenous levels of total ATP5I.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP5I, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5I

Target: ATP5I (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit E (ATP5I))

Alternative Name: ATP5I

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.

Gene: ATP5I

Molecular Weight: 8 kDa

Gene ID: 521

UniProt: P56385

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process