USP44 antibody (Internal Region)

Catalog No. ABIN6258564

The Rabbit Polyclonal anti-USP44 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect USP44 in samples from Human and Mouse.

Quick Overview for USP44 antibody (Internal Region) (ABIN6258564)

Target: USP44 (Ubiquitin Specific Peptidase 44 (USP44))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP44 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-USP44 Antibody

Binding Specificity: Internal Region

Specificity: USP44 Antibody detects endogenous levels of total USP44.

Predicted Reactivity: Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human USP44, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for USP44

Target: USP44 (Ubiquitin Specific Peptidase 44 (USP44))

Alternative Name: USP44

Background:

Description: Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.

Gene: USP44

Molecular Weight: 81 kDa

Gene ID: 84101

UniProt: Q9H0E7

Pathways: M Phase