YWHAE antibody (C-Term)

Catalog No. ABIN6258570

The Rabbit Polyclonal anti-YWHAE antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect YWHAE in samples from Human, Mouse and Rat.

Quick Overview for YWHAE antibody (C-Term) (ABIN6258570)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-YWHAE Antibody

Binding Specificity: C-Term

Specificity: 14-3-3 epsilon Antibody detects endogenous levels of total 14-3-3 epsilon.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human 14-3-3 epsilon, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE

Background:

Description: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).

Gene: YWHAE

Molecular Weight: 29 kDa

Gene ID: 7531

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase