FZD1 antibody (N-Term)

Catalog No. ABIN6258786

The Rabbit Polyclonal anti-FZD1 antibody is suitable to detect FZD1 in samples from Human and Rat. It has been validated for WB, ELISA, IHC, ICC and IF.

Quick Overview for FZD1 antibody (N-Term) (ABIN6258786)

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-FZD1 Antibody

Binding Specificity: N-Term

Specificity: FZD1 Antibody detects endogenous levels of total FZD1.

Predicted Reactivity: Pig,Zebrafish,Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human FZD1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1

Background:

Description: Receptor for Wnt proteins (PubMed:10557084). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (PubMed:10557084). Contradictory results showing activation by WNT7B have been described for mouse (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:10557084). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:10557084). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).

Gene: FZD1

Molecular Weight: 71 kDa

Gene ID: 8321

UniProt: Q9UP38

Pathways: WNT Signaling, Asymmetric Protein Localization