MHC Class II RT-1B antibody

Catalog No. ABIN625901

The Mouse Monoclonal anti-MHC Class II RT-1B antibody has been validated for FACS, IHC (fro), IHC (p), WB and IHC. It is suitable to detect MHC Class II RT-1B in samples from Rat and Mouse.

Quick Overview for MHC Class II RT-1B antibody (ABIN625901)

Target: MHC Class II RT-1B

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II RT-1B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: OX-6

Product Details anti-MHC Class II RT-1B Antibody

Brand: IHC-plus™

Specificity: Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

Purification: Protein G purified

Immunogen: Rat thymocyte membrane glycoprotein.

Type of Immunogen: Membrane extract

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer  pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MHC Class II RT-1B

Target: MHC Class II RT-1B