MSH3 antibody (N-Term)

Catalog No. ABIN6259073

This Rabbit Polyclonal antibody specifically detects MSH3 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MSH3 antibody (N-Term) (ABIN6259073)

Target: MSH3 (MutS Homolog 3 (MSH3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MSH3 Antibody

Binding Specificity: N-Term

Specificity: MSH3 Antibody detects endogenous levels of total MSH3.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MSH3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MSH3

Target: MSH3 (MutS Homolog 3 (MSH3))

Alternative Name: MSH3

Background:

Description: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Gene: MSH3

Molecular Weight: 127 kDa

Gene ID: 4437

UniProt: P20585

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response