PARD3 antibody (C-Term)

Catalog No. ABIN6259144

This anti-PARD3 antibody is a Rabbit Polyclonal antibody detecting PARD3 in WB, ELISA, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for PARD3 antibody (C-Term) (ABIN6259144)

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PARD3 Antibody

Binding Specificity: C-Term

Specificity: PARD3 Antibody detects endogenous levels of total PARD3.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PARD3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PARD3

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: PARD3

Background:

Description: Adapter protein involved in asymmetrical cell division and cell polarization processes (PubMed:27925688, PubMed:10954424). Seems to play a central role in the formation of epithelial tight junctions (PubMed:27925688). Targets the phosphatase PTEN to cell junctions (By similarity). Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:10934474). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (PubMed:19812038, PubMed:27925688).

Gene: PARD3

Molecular Weight: 151 kDa

Gene ID: 56288

UniProt: Q8TEW0

Pathways: Cell-Cell Junction Organization