PARD6B antibody (Internal Region)

Catalog No. ABIN6259145

This Rabbit Polyclonal antibody specifically detects PARD6B in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for PARD6B antibody (Internal Region) (ABIN6259145)

Target: PARD6B (Par-6 Partitioning Defective 6 Homolog beta (PARD6B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD6B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PARD6B Antibody

Binding Specificity: Internal Region

Specificity: PARD6B Antibody detects endogenous levels of total PARD6B.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PARD6B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PARD6B

Target: PARD6B (Par-6 Partitioning Defective 6 Homolog beta (PARD6B))

Alternative Name: PARD6B

Background:

Description: Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Gene: PARD6B

Molecular Weight: 38 kDa

Gene ID: 84612

UniProt: Q9BYG5

Pathways: Cell-Cell Junction Organization