SRSF3 antibody (C-Term)

Catalog No. ABIN6259187

This Rabbit Polyclonal antibody specifically detects SRSF3 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for SRSF3 antibody (C-Term) (ABIN6259187)

Target: SRSF3 (serine/arginine-Rich Splicing Factor 3 (SRSF3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRSF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SRSF3 Antibody

Binding Specificity: C-Term

Specificity: SFRS3 Antibody detects endogenous levels of total SFRS3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SFRS3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SRSF3

Target: SRSF3 (serine/arginine-Rich Splicing Factor 3 (SRSF3))

Alternative Name: SRSF3

Background:

Description: Splicing factor that specifically promotes exon-inclusion during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). Also functions as export adapter involved in mRNA nuclear export such as of histone H2A (PubMed:11336712, PubMed:18364396). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway), enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). RNA-binding is semi-sequence specific (PubMed:17036044).

Gene: SRSF3

Molecular Weight: 25 kDa

Gene ID: 6428

UniProt: P84103