NLE1 antibody (Internal Region)

Catalog No. ABIN6259478

This Rabbit Polyclonal antibody specifically detects NLE1 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for NLE1 antibody (Internal Region) (ABIN6259478)

Target: NLE1 (Notchless Homolog 1 (NLE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NLE1 Antibody

Binding Specificity: Internal Region

Specificity: NLE1 Antibody detects endogenous levels of total NLE1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NLE1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NLE1

Target: NLE1 (Notchless Homolog 1 (NLE1))

Alternative Name: NLE1

Background:

Description: Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).

Gene: NLE1

Molecular Weight: 48 kDa

Gene ID: 54475

UniProt: Q9NVX2