WEE2 antibody

Catalog No. ABIN6259536

The Rabbit Polyclonal anti-WEE2 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect WEE2 in samples from Human and Mouse.

Quick Overview for WEE2 antibody (ABIN6259536)

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-WEE2 Antibody

Specificity: WEE2 Antibody detects endogenous levels of total WEE2

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for WEE2

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Alternative Name: WEE2

Background:

Description: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).

Gene: WEE2

Molecular Weight: 60 kDa

Gene ID: 494551

UniProt: P0C1S8

Pathways: M Phase