alpha 1 Adrenergic Receptor antibody (Internal Region)

Catalog No. ABIN6259548

The Rabbit Polyclonal anti-alpha 1 Adrenergic Receptor antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect alpha 1 Adrenergic Receptor in samples from Human, Rat and Mouse.

Quick Overview for alpha 1 Adrenergic Receptor antibody (Internal Region) (ABIN6259548)

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 1 Adrenergic Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-alpha 1 Adrenergic Receptor Antibody

Binding Specificity: Internal Region

Specificity: ADRA1A Antibody detects endogenous levels of total ADRA1A.

Predicted Reactivity: Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ADRA1A, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for alpha 1 Adrenergic Receptor

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Alternative Name: ADRA1A

Background:

Description: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

Gene: ADRA1A

Molecular Weight: 51 kDa

Gene ID: 148

UniProt: P35348

Pathways: AMPK Signaling