alpha 1 Adrenergic Receptor antibody (2nd Extracellular Loop, C176S)

Catalog No. ABIN7886053

The Rabbit Polyclonal anti-alpha 1 Adrenergic Receptor antibody (ABIN7886053) specifically detects alpha 1 Adrenergic Receptor in WB, IF, IHC, FACS and LCI. The antibody is reactive with Human samples.

Quick Overview for alpha 1 Adrenergic Receptor antibody (2nd Extracellular Loop, C176S) (ABIN7886053)

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 1 Adrenergic Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-alpha 1 Adrenergic Receptor Antibody

Binding Specificity: 2nd Extracellular Loop, AA 171-183, C176S

Purpose: A Rabbit Polyclonal Antibody to α1A-Adrenergic Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat,mouse,bovine - 12,13 amino acid residues identical

Characteristics: Anti-alpha1A-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the human α1A-adrenergic receptor. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (ABIN7042908, ABIN7043908 and ABIN7043909) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α1A-adrenoceptor from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human alpha1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S) 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234685)

Blocking Peptide: (ABIN7234685)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for alpha 1 Adrenergic Receptor

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Alternative Name: ADRA1A

Background:

Synonyms: Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, α1A-AR, ADRA1A

Description: Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4The α1A-ARs have at least nine known alternative spliced isoforms. They are the most abundant adrenergic receptor in the brain, and are considered postsynaptic receptors. α1A-ARs are also expressed in the heart, liver and prostate.

Gene ID: 148

NCBI Accession: NM_033303

UniProt: P35348

Pathways: AMPK Signaling