YOD1 antibody (Internal Region)

Catalog No. ABIN6259576

The Rabbit Polyclonal anti-YOD1 antibody (ABIN6259576) specifically detects YOD1 in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for YOD1 antibody (Internal Region) (ABIN6259576)

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-YOD1 Antibody

Binding Specificity: Internal Region

Specificity: YOD1 Antibody detects endogenous levels of total YOD1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human YOD1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for YOD1

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Alternative Name: YOD1

Background:

Description: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation (PubMed:27753622).

Gene: YOD1

Molecular Weight: 35 kDa

Gene ID: 55432

UniProt: Q5VVQ6

Pathways: ER-Nucleus Signaling