ZAK antibody (N-Term)

Catalog No. ABIN6259601

This Rabbit Polyclonal antibody specifically detects ZAK in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for ZAK antibody (N-Term) (ABIN6259601)

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ZAK Antibody

Binding Specificity: N-Term

Specificity: ZAK Antibody detects endogenous levels of total ZAK.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ZAK, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ZAK

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Alternative Name: MAP3K20

Background:

Description: Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636).

Gene: MAP3K20

Molecular Weight: 91 kDa

Gene ID: 51776

UniProt: Q9NYL2