ARL3 antibody (C-Term)

Catalog No. ABIN6260040

The Rabbit Polyclonal anti-ARL3 antibody has been validated for WB. It is suitable to detect ARL3 in samples from Human, Mouse and Rat.

Quick Overview for ARL3 antibody (C-Term) (ABIN6260040)

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARL3 Antibody

Binding Specificity: C-Term

Specificity: ARL3 Antibody detects endogenous levels of total ARL3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ARL3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARL3

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Alternative Name: ARL3

Background:

Description: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.

Gene: ARL3

Molecular Weight: 20 kDa

Gene ID: 403

UniProt: P36405