ASPM antibody (Internal Region)

Catalog No. ABIN6260082

The Rabbit Polyclonal anti-ASPM antibody is suitable to detect ASPM in samples from Human and Rat. It has been validated for ELISA, IHC, IF, WB and ICC.

Quick Overview for ASPM antibody (Internal Region) (ABIN6260082)

Target: ASPM (Asp (Abnormal Spindle) Homolog, Microcephaly Associated (ASPM))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASPM antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-ASPM Antibody

Binding Specificity: Internal Region

Specificity: ASPM Antibody detects endogenous levels of total ASPM.

Predicted Reactivity: Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ASPM, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ASPM

Target: ASPM (Asp (Abnormal Spindle) Homolog, Microcephaly Associated (ASPM))

Alternative Name: ASPM

Background:

Description: Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex (PubMed:28436967). May have a preferential role in regulating neurogenesis.

Gene: ASPM

Molecular Weight: 410 kDa

Gene ID: 259266

UniProt: Q8IZT6