ATPBD3 antibody (C-Term)

Catalog No. ABIN6260142

The Rabbit Polyclonal anti-ATPBD3 antibody is suitable to detect ATPBD3 in samples from Human. It has been validated for WB, ELISA, ICC and IF.

Quick Overview for ATPBD3 antibody (C-Term) (ABIN6260142)

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATPBD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ATPBD3 Antibody

Binding Specificity: C-Term

Specificity: ATPBD3 Antibody detects endogenous levels of total ATPBD3.

Predicted Reactivity: Pig,Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATPBD3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATPBD3

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Alternative Name: CTU1

Background:

Description: Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

Gene: CTU1

Molecular Weight: 36kDa

Gene ID: 90353

UniProt: Q7Z7A3