EN1 antibody (AA 266-392)

Catalog No. ABIN626042

The Mouse Monoclonal anti-EN1 antibody is suitable to detect EN1 in samples from Human. It has been validated for WB, ELISA, IHC (p) and IHC.

Quick Overview for EN1 antibody (AA 266-392) (ABIN626042)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Clone: 1F5

Product Details anti-EN1 Antibody

Binding Specificity: AA 266-392

Specificity: Human Engrailed Homolog 1

Purification: Purified from ascites by Protein A

Immunogen: EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot performed with the recombinant protein used as the immunogen.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in Ascites.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1 / Engrailed

Background: Name/Gene ID: EN1

Synonyms: EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1

Gene ID: 2019

NCBI Accession: NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis