Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (N-Term) antibody

Catalog No. ABIN6260515

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IHC and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (N-Term) antibody (ABIN6260515)

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details

Binding Specificity: N-Term

Specificity: CBLB Antibody detects endogenous levels of total CBLB.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CBLB, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Alternative Name: CBLB

Background:

Description: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

Gene: CBLB

Molecular Weight: 109kDa

Gene ID: 868

UniProt: Q13191