CHD5 antibody (Internal Region)

Catalog No. ABIN6260796

The Rabbit Polyclonal anti-CHD5 antibody has been validated for ELISA, WB, IF and ICC. It is suitable to detect CHD5 in samples from Human.

Quick Overview for CHD5 antibody (Internal Region) (ABIN6260796)

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CHD5 Antibody

Binding Specificity: Internal Region

Specificity: CHD5 Antibody detects endogenous levels of total CHD5.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CHD5, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:200-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CHD5

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Alternative Name: CHD5

Background:

Description: Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.

Gene: CHD5

Molecular Weight: 223 kDa

Gene ID: 26038

UniProt: Q8TDI0