DGKH antibody (Internal Region)

Catalog No. ABIN6261284

This Rabbit Polyclonal antibody specifically detects DGKH in ELISA, IHC, WB, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DGKH antibody (Internal Region) (ABIN6261284)

Target: DGKH (Diacylglycerol Kinase, eta (DGKH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKH antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DGKH Antibody

Binding Specificity: Internal Region

Specificity: DGKH Antibody detects endogenous levels of total DGKH.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DGKH, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DGKH

Target: DGKH (Diacylglycerol Kinase, eta (DGKH))

Alternative Name: DGKH

Background:

Description: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

Gene: DGKH

Molecular Weight: 134kDa

Gene ID: 160851

UniProt: Q86XP1