DNAJA1 antibody (C-Term)

Catalog No. ABIN6261327

The Rabbit Polyclonal anti-DNAJA1 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect DNAJA1 in samples from Human, Mouse and Rat.

Quick Overview for DNAJA1 antibody (C-Term) (ABIN6261327)

Target: DNAJA1 (DnaJ (Hsp40) Homolog, Subfamily A, Member 1 (DNAJA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DNAJA1 Antibody

Binding Specificity: C-Term

Specificity: DNAJA1 Antibody detects endogenous levels of total DNAJA1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DNAJA1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DNAJA1

Target: DNAJA1 (DnaJ (Hsp40) Homolog, Subfamily A, Member 1 (DNAJA1))

Alternative Name: DNAJA1

Background:

Description: Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).

Gene: DNAJA1

Molecular Weight: 44 kDa

Gene ID: 3301

UniProt: P31689

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway