DNAJB14 antibody (C-Term)

Catalog No. ABIN6261329

This Rabbit Polyclonal antibody specifically detects DNAJB14 in WB, IF, IHC and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DNAJB14 antibody (C-Term) (ABIN6261329)

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-DNAJB14 Antibody

Binding Specificity: C-Term

Specificity: DNAJB14 Antibody detects endogenous levels of total DNAJB14.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DNAJB14, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DNAJB14

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Alternative Name: DNAJB14

Background:

Description: Acts as a co-chaperone with HSPA8/Hsc70, required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:24732912). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:24732912). Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus, the role of these structures, named DJANGOs, is still unclear (PubMed:24732912).

Gene: DNAJB14

Molecular Weight: 43 kDa

Gene ID: 79982

UniProt: Q8TBM8