DNAJC3 antibody (C-Term)

Catalog No. ABIN6261337

This anti-DNAJC3 antibody is a Rabbit Polyclonal antibody detecting DNAJC3 in ELISA, WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for DNAJC3 antibody (C-Term) (ABIN6261337)

Target: DNAJC3 (DnaJ (Hsp40) Homolog, Subfamily C, Member 3 (DNAJC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DNAJC3 Antibody

Binding Specificity: C-Term

Specificity: DNAJC3 Antibody detects endogenous levels of total DNAJC3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DNAJC3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DNAJC3

Target: DNAJC3 (DnaJ (Hsp40) Homolog, Subfamily C, Member 3 (DNAJC3))

Alternative Name: DNAJC3

Background:

Description: Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

Gene: DNAJC3

Molecular Weight: 54 kDa

Gene ID: 5611

UniProt: Q13217

Pathways: ER-Nucleus Signaling