SHFM1 antibody

Catalog No. ABIN6261375

The Rabbit Polyclonal anti-SHFM1 antibody is suitable to detect SHFM1 in samples from Human and Rat. It has been validated for IHC and WB.

Quick Overview for SHFM1 antibody (ABIN6261375)

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHFM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-SHFM1 Antibody

Specificity: DSS1 antibody detects endogenous levels of DSS1.

Cross-Reactivity: Human, Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DSS1.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for SHFM1

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Alternative Name: DSS1

Background:

Description: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:15117943). Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.

Gene: SEM1

Molecular Weight: 8-10 kDa

Gene ID: 7979

UniProt: P60896