EDEM1 antibody (C-Term)

Catalog No. ABIN6261429

The Rabbit Polyclonal anti-EDEM1 antibody has been validated for WB and ELISA. It is suitable to detect EDEM1 in samples from Human and Mouse.

Quick Overview for EDEM1 antibody (C-Term) (ABIN6261429)

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDEM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EDEM1 Antibody

Binding Specificity: C-Term

Specificity: EDEM1 Antibody detects endogenous levels of total EDEM1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EDEM1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EDEM1

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Alternative Name: EDEM1

Background:

Description: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.

Gene: EDEM1

Molecular Weight: 74 kDa

Gene ID: 9695

UniProt: Q92611

Pathways: ER-Nucleus Signaling