EIF3S1 antibody (Internal Region)

Catalog No. ABIN6261484

This anti-EIF3S1 antibody is a Rabbit Polyclonal antibody detecting EIF3S1 in WB, ELISA, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for EIF3S1 antibody (Internal Region) (ABIN6261484)

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3S1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EIF3S1 Antibody

Binding Specificity: Internal Region

Specificity: EIF3J Antibody detects endogenous levels of total EIF3J.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3J, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF3S1

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Alternative Name: EIF3J

Background:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3J

Molecular Weight: 29 kDa

Gene ID: 8669

UniProt: O75822

Pathways: Ribonucleoprotein Complex Subunit Organization