ERCC2 antibody (C-Term)

Catalog No. ABIN6261589

This anti-ERCC2 antibody is a Rabbit Polyclonal antibody detecting ERCC2 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for ERCC2 antibody (C-Term) (ABIN6261589)

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ERCC2 Antibody

Binding Specificity: C-Term

Specificity: ERCC2 Antibody detects endogenous levels of total ERCC2.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ERCC2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ERCC2

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Alternative Name: ERCC2

Background:

Description: ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

Gene: ERCC2

Molecular Weight: 87kDa

Gene ID: 2068

UniProt: P18074

Pathways: DNA Damage Repair