ETAA1 antibody (Internal Region)

Catalog No. ABIN6261612

The Rabbit Polyclonal anti-ETAA1 antibody is suitable to detect ETAA1 in samples from Human and Mouse. It has been validated for WB, ELISA, ICC and IF.

Quick Overview for ETAA1 antibody (Internal Region) (ABIN6261612)

Target: ETAA1 (Ewing Tumor-Associated Antigen 1 (ETAA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETAA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ETAA1 Antibody

Binding Specificity: Internal Region

Specificity: ETAA1 Antibody detects endogenous levels of total ETAA1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ETAA1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ETAA1

Target: ETAA1 (Ewing Tumor-Associated Antigen 1 (ETAA1))

Alternative Name: ETAA1

Background:

Description: Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity (PubMed:27601467, PubMed:27723720, PubMed:27723717). Recruited to stalled replication forks via interaction with the RPA complex and directly stimulates ATR kinase activity independently of TOPBP1 (PubMed:27723720, PubMed:27723717). Probably only regulates a subset of ATR targets (PubMed:27723720, PubMed:27723717).

Gene: ETAA1

Molecular Weight: 103 kDa

Gene ID: 54465

UniProt: Q9NY74