GCLM antibody (N-Term)

Catalog No. ABIN6261915

The Rabbit Polyclonal anti-GCLM antibody has been validated for WB, IHC and ELISA. It is suitable to detect GCLM in samples from Human, Mouse, Rat and Pig. There are 2+ publications available.

Quick Overview for GCLM antibody (N-Term) (ABIN6261915)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-GCLM Antibody

Binding Specificity: N-Term

Specificity: GCLM Antibody detects endogenous levels of total GCLM.

Predicted Reactivity: Pig,Bovine,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GCLM, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-GCLM antibody (ABIN6261915)

Zou, Wang, Peng, Wei: "Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5987183, (2017) (PubMed).

Li, Sun, Liu, Zhao, Shao: "Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 102, pp. 93-101, (2017) (PubMed).

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background:

Gene: GCLM

Molecular Weight: 30kDa

Gene ID: 2730

UniProt: P48507