GRP78 antibody

Catalog No. ABIN6262100

This Rabbit Polyclonal antibody specifically detects GRP78 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Rat, Mouse and Monkey and has been mentioned in 4+ publications.

Quick Overview for GRP78 antibody (ABIN6262100)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GRP78 Antibody

Specificity: GRP78 Antibody detects endogenous levels of total GRP78

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GRP78

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:50-1:200,IF 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-GRP78 antibody (ABIN6262100)

Tang, Peng, Huang, Xie, Chen, Shen, Gao, You, Xie, Chen: "Neoisoliquiritigenin Inhibits Tumor Progression by Targeting GRP78-β- catenin Signaling in Breast Cancer." in: Current cancer drug targets, Vol. 18, Issue 4, pp. 390-399, (2018) (PubMed).

Tan, Li, Zhai, Liu, Li, Yokota, Zhang: "Effects of knee loading on obesity-related non-alcoholic fatty liver disease in an ovariectomized mouse model with high-fat diet." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 48, Issue 10, pp. 839-849, (2018) (PubMed).

Liu, Shen, Wang, Liu, Zheng, Luo: "EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes." in: Journal of cellular biochemistry, (2018) (PubMed).

Li, Yang, Li, Liu, Wang, Guo, Tan, Gao, Zhao, Zhang, Gou, Yokota, Zhang: "Role of endoplasmic reticulum stress in disuse osteoporosis." in: Bone, Vol. 97, pp. 2-14, (2017) (PubMed).

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: GRP78

Background:

Description: Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).

Gene: HSPA5

Molecular Weight: 78 kDa

Gene ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling