HFE antibody (Internal Region)

Catalog No. ABIN6262227

The Rabbit Polyclonal anti-HFE antibody has been validated for WB, ELISA, IF, IHC and ICC. It is suitable to detect HFE in samples from Human, Mouse and Rat.

Quick Overview for HFE antibody (Internal Region) (ABIN6262227)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HFE Antibody

Binding Specificity: Internal Region

Specificity: HFE Antibody detects endogenous levels of total HFE.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HFE, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background:

Description: Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.

Gene: HFE

Molecular Weight: 40kDa

Gene ID: 3077

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process