EPAS1 antibody (Internal Region)

Catalog No. ABIN6262236

The Rabbit Polyclonal anti-EPAS1 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect EPAS1 in samples from Human, Rat and Mouse.

Quick Overview for EPAS1 antibody (Internal Region) (ABIN6262236)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-EPAS1 Antibody

Binding Specificity: Internal Region

Specificity: HIF-2-alpha Antibody detects endogenous levels of total HIF-2-alpha.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HIF-2-alpha, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1

Background:

Description: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Gene: EPAS1

Molecular Weight: 96kDa

Gene ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect