IDH3B antibody (N-Term)

Catalog No. ABIN6262446

The Rabbit Polyclonal anti-IDH3B antibody has been validated for WB and ELISA. It is suitable to detect IDH3B in samples from Human, Mouse and Rat.

Quick Overview for IDH3B antibody (N-Term) (ABIN6262446)

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IDH3B Antibody

Binding Specificity: N-Term

Specificity: IDH3B Antibody detects endogenous levels of total IDH3B.

Predicted Reactivity: Pig,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IDH3B, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for IDH3B

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Alternative Name: IDH3B

Background:

Description: Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Gene: IDH3B

Molecular Weight: 42 kDa

Gene ID: 3420

UniProt: O43837