MED29 antibody (Internal Region)

Catalog No. ABIN6263195

This Rabbit Polyclonal antibody specifically detects MED29 in WB, ELISA, IF, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MED29 antibody (Internal Region) (ABIN6263195)

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-MED29 Antibody

Binding Specificity: Internal Region

Specificity: MED29 Antibody detects endogenous levels of total MED29.

Predicted Reactivity: Bovine,Horse,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MED29, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED29

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Alternative Name: MED29

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene: MED29

Molecular Weight: 21 kDa

Gene ID: 55588

UniProt: Q9NX70

Pathways: Regulation of Lipid Metabolism by PPARalpha