MED29 antibody (Middle Region)

Catalog No. ABIN2789154

This Rabbit Polyclonal antibody specifically detects MED29 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for MED29 antibody (Middle Region) (ABIN2789154)

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED29 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED29 Antibody

Binding Specificity: Middle Region

Purpose: Med29 Antibody - middle region

Sequence: LQRFDKCLEE FYALCDQLEL CLRLAHECLS QSCDSAKHSP TLVPTATKPD

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against Med29. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Med29

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP62470-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED29

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Alternative Name: Med29

Background:

Targeted Subunit: 29

Background Information: Med29 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Name: mediator complex subunit 29

Alternative Symbols: I, Ixl, AU020902, 2810405O22Rik

Protein Name: Mediator of RNA polymerase II transcription subunit 29

Molecular Weight: 21kDa

Gene ID: 67224

NCBI Accession: NP_080318

UniProt: Q9DB91

Pathways: Regulation of Lipid Metabolism by PPARalpha