MED30 antibody (Internal Region)

Catalog No. ABIN6263196

The Rabbit Polyclonal anti-MED30 antibody has been validated for WB and ELISA. It is suitable to detect MED30 in samples from Human, Mouse and Rat.

Quick Overview for MED30 antibody (Internal Region) (ABIN6263196)

Target: MED30 (Mediator Complex Subunit 30 (MED30))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED30 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED30 Antibody

Binding Specificity: Internal Region

Specificity: MED30 Antibody detects endogenous levels of total MED30.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MED30, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED30

Target: MED30 (Mediator Complex Subunit 30 (MED30))

Alternative Name: MED30

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene: MED30

Molecular Weight: 20 kDa

Gene ID: 90390

UniProt: Q96HR3

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha